Review



ihc staining images of opn  (Human Protein Atlas)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Human Protein Atlas ihc staining images of opn
    High <t>OPN</t> expression in HCC. ( a ) The mRNA level of SPP1 in different types of tumor tissues and normal tissues in TIMER database. ( b ) Representative images of OPN protein level in HCC detected <t>by</t> <t>IHC</t> staining in HPA database (×400 magnification). ( c ) Comparison of OPN protein expression between HCC and normal tissues in CPTAC database. A two-sided p value was calculated using a Student’s t -test. The box plot indicates the median (center), 25th and 75th percentiles (box boundaries), and the minimum and maximum (whiskers). ( d ) Single-cell RNA sequencing localization analysis of SPP1. (Left) t-SNE plot of three main cell types (Macrophages, dendritic cells, and B cells) in hepatic monocyte-derived macrophages. (Right) SPP1 was enriched in macrophages clusters.
    Ihc Staining Images Of Opn, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ihc+staining+images+of+opn/ihc+staining+images+of+opn/pmc12007010-112-5-16
    Average 90 stars, based on 1 article reviews
    ihc staining images of opn - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Serum Osteopontin Enhances Hepatocellular Carcinoma Diagnosis and Predicts Anti-PD-L1 Immunotherapy Benefit"

    Article Title: Serum Osteopontin Enhances Hepatocellular Carcinoma Diagnosis and Predicts Anti-PD-L1 Immunotherapy Benefit

    Journal: Journal of Hepatocellular Carcinoma

    doi: 10.2147/JHC.S514144

    High OPN expression in HCC. ( a ) The mRNA level of SPP1 in different types of tumor tissues and normal tissues in TIMER database. ( b ) Representative images of OPN protein level in HCC detected by IHC staining in HPA database (×400 magnification). ( c ) Comparison of OPN protein expression between HCC and normal tissues in CPTAC database. A two-sided p value was calculated using a Student’s t -test. The box plot indicates the median (center), 25th and 75th percentiles (box boundaries), and the minimum and maximum (whiskers). ( d ) Single-cell RNA sequencing localization analysis of SPP1. (Left) t-SNE plot of three main cell types (Macrophages, dendritic cells, and B cells) in hepatic monocyte-derived macrophages. (Right) SPP1 was enriched in macrophages clusters.
    Figure Legend Snippet: High OPN expression in HCC. ( a ) The mRNA level of SPP1 in different types of tumor tissues and normal tissues in TIMER database. ( b ) Representative images of OPN protein level in HCC detected by IHC staining in HPA database (×400 magnification). ( c ) Comparison of OPN protein expression between HCC and normal tissues in CPTAC database. A two-sided p value was calculated using a Student’s t -test. The box plot indicates the median (center), 25th and 75th percentiles (box boundaries), and the minimum and maximum (whiskers). ( d ) Single-cell RNA sequencing localization analysis of SPP1. (Left) t-SNE plot of three main cell types (Macrophages, dendritic cells, and B cells) in hepatic monocyte-derived macrophages. (Right) SPP1 was enriched in macrophages clusters.

    Techniques Used: Expressing, Immunohistochemistry, Comparison, RNA Sequencing, Derivative Assay

    Diagnostic value of serum OPN for HCC. ( a – d ) Violin plots of serum OPN levels in healthy participants, CLD patients, and HCC patients ( a ), in early-stage HCC patients and advanced-stage HCC patients ( b ), in CLD patients and early-stage HCC patients ( c ), in CLD patients and AFP-negative HCC patients ( d ). ( e – g ) ROC curve analysis reveals the diagnostic potential of serum OPN in 178 HCC patients and 138 controls ( e ), in 98 early-stage HCC patients and 53 chronic liver disease patients ( f ), in 101 AFP-negative HCC patients and 53 chronic liver disease patients ( g ). ( h–j ) The waterfall plot illustrates the risk probability distribution between serum samples of patients with HCC (n = 178) and the controls (n = 138) in the whole cohort ( h ), patients with early-stage HCC (n = 98) and the chronic liver disease (n = 53) ( i ), patients with AFP-negative HCC (n = 101) and the chronic liver disease (n = 53) ( j ). ( k ) The rate of positive results for AFP, OPN, or both, in all patients with HCC, for OPN in AFP-negative HCC, and for OPN in early-stage HCC, respectively. ( l ) Comparison of ROC of four diagnostic models for distinguishing HCC from whole controls.
    Figure Legend Snippet: Diagnostic value of serum OPN for HCC. ( a – d ) Violin plots of serum OPN levels in healthy participants, CLD patients, and HCC patients ( a ), in early-stage HCC patients and advanced-stage HCC patients ( b ), in CLD patients and early-stage HCC patients ( c ), in CLD patients and AFP-negative HCC patients ( d ). ( e – g ) ROC curve analysis reveals the diagnostic potential of serum OPN in 178 HCC patients and 138 controls ( e ), in 98 early-stage HCC patients and 53 chronic liver disease patients ( f ), in 101 AFP-negative HCC patients and 53 chronic liver disease patients ( g ). ( h–j ) The waterfall plot illustrates the risk probability distribution between serum samples of patients with HCC (n = 178) and the controls (n = 138) in the whole cohort ( h ), patients with early-stage HCC (n = 98) and the chronic liver disease (n = 53) ( i ), patients with AFP-negative HCC (n = 101) and the chronic liver disease (n = 53) ( j ). ( k ) The rate of positive results for AFP, OPN, or both, in all patients with HCC, for OPN in AFP-negative HCC, and for OPN in early-stage HCC, respectively. ( l ) Comparison of ROC of four diagnostic models for distinguishing HCC from whole controls.

    Techniques Used: Diagnostic Assay, Comparison

    Serum OPN as an independent predictor for HCC survival. ( a ) The inter-relationship between each clinical parameters in HCC patients. Values denote the Pearson correlation coefficients; values closer to 1 indicate a better correlation (* p < 0.05; ** p < 0.001). ( b ) Optimal cutoff value of serum OPN was determined by the “surv_cutpoint” function. ( c ) Forest plot of multivariate Cox regression analysis showing serum OPN level, hepatectomy, tumor size, Child-Pugh grade, and BCLC stage as independent prognostic factors for OS. ( d ) Forest plot of multivariate Cox regression analysis showing serum OPN level, Child-Pugh grade, and BCLC stage as independent prognostic factors for DFS. ( e ) Alluvial diagram showing the interrelationship between AFP status, OPN groups, and OS status in HCC. ( f ) Alluvial diagram showing the interrelationship between OPN groups, BCLC stage, and recurrence status in HCC patients.
    Figure Legend Snippet: Serum OPN as an independent predictor for HCC survival. ( a ) The inter-relationship between each clinical parameters in HCC patients. Values denote the Pearson correlation coefficients; values closer to 1 indicate a better correlation (* p < 0.05; ** p < 0.001). ( b ) Optimal cutoff value of serum OPN was determined by the “surv_cutpoint” function. ( c ) Forest plot of multivariate Cox regression analysis showing serum OPN level, hepatectomy, tumor size, Child-Pugh grade, and BCLC stage as independent prognostic factors for OS. ( d ) Forest plot of multivariate Cox regression analysis showing serum OPN level, Child-Pugh grade, and BCLC stage as independent prognostic factors for DFS. ( e ) Alluvial diagram showing the interrelationship between AFP status, OPN groups, and OS status in HCC. ( f ) Alluvial diagram showing the interrelationship between OPN groups, BCLC stage, and recurrence status in HCC patients.

    Techniques Used:

    Correlation Between Serum OPN and Clinicopathologic Factors in HCC Patients
    Figure Legend Snippet: Correlation Between Serum OPN and Clinicopathologic Factors in HCC Patients

    Techniques Used:

    Prognostic value of OPN in patients with HCC. ( a and b ) In the whole HCC cohort, patients with low levels of serum OPN had longer overall survival ( a ) and disease-free survival (b). ( c – i ) The prognostic value of serum OPN in different subgroups regarding overall survival, including early-stage ( c ), AFP-negative ( d ), Child-Pugh grade A ( e ), hepatectomy ( f ), tumor size ≤ 5 cm ( g ), MVI absent ( h ), ALBI grade 1 ( i ).
    Figure Legend Snippet: Prognostic value of OPN in patients with HCC. ( a and b ) In the whole HCC cohort, patients with low levels of serum OPN had longer overall survival ( a ) and disease-free survival (b). ( c – i ) The prognostic value of serum OPN in different subgroups regarding overall survival, including early-stage ( c ), AFP-negative ( d ), Child-Pugh grade A ( e ), hepatectomy ( f ), tumor size ≤ 5 cm ( g ), MVI absent ( h ), ALBI grade 1 ( i ).

    Techniques Used:

    Serum OPN is a predictor of anti-PD-L1 immunotherapy benefit in patients with HCC. ( a and b ) Immunotherapy significantly prolonged the OS ( a ) and DFS ( b ) of HCC patients with high OPN levels. ( c and d ) HCC patients with low OPN levels did not derived a significant survival benefit from immunotherapy. ( e and f) OS ( e ) and DFS ( f ) of OPN high and OPN low patients in the immunotherapy group were not significantly different. ( g and h ) OPN high patients exhibited a significantly shorter OS ( g ) and DFS ( h ) than OPN low patients in the group without immunotherapy.
    Figure Legend Snippet: Serum OPN is a predictor of anti-PD-L1 immunotherapy benefit in patients with HCC. ( a and b ) Immunotherapy significantly prolonged the OS ( a ) and DFS ( b ) of HCC patients with high OPN levels. ( c and d ) HCC patients with low OPN levels did not derived a significant survival benefit from immunotherapy. ( e and f) OS ( e ) and DFS ( f ) of OPN high and OPN low patients in the immunotherapy group were not significantly different. ( g and h ) OPN high patients exhibited a significantly shorter OS ( g ) and DFS ( h ) than OPN low patients in the group without immunotherapy.

    Techniques Used: Derivative Assay



    Similar Products

    ihc staining images of opn  (Human Protein Atlas)
    90
    Human Protein Atlas ihc staining images of opn
    High <t>OPN</t> expression in HCC. ( a ) The mRNA level of SPP1 in different types of tumor tissues and normal tissues in TIMER database. ( b ) Representative images of OPN protein level in HCC detected <t>by</t> <t>IHC</t> staining in HPA database (×400 magnification). ( c ) Comparison of OPN protein expression between HCC and normal tissues in CPTAC database. A two-sided p value was calculated using a Student’s t -test. The box plot indicates the median (center), 25th and 75th percentiles (box boundaries), and the minimum and maximum (whiskers). ( d ) Single-cell RNA sequencing localization analysis of SPP1. (Left) t-SNE plot of three main cell types (Macrophages, dendritic cells, and B cells) in hepatic monocyte-derived macrophages. (Right) SPP1 was enriched in macrophages clusters.
    Ihc Staining Images Of Opn, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ihc+staining+images+of+opn/ihc+staining+images+of+opn/pmc12007010-112-5-16
    Average 90 stars, based on 1 article reviews
    ihc staining images of opn - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    immunohistochemistry (ihc) staining images of col3a1, spp1, and thbs2  (Human Protein Atlas)
    90
    Human Protein Atlas immunohistochemistry (ihc) staining images of col3a1, spp1, and thbs2
    Clinical pathological indicators and prognostic characteristics of elevated extracellular matrix (ECM) subtype genes: Analysis of the correlations between ECM subtype gene expression and survival and clinical characteristics. (A) Survival analysis for the ECM subtype genes COMP, SPP1, <t>THBS2,</t> COL1A1, COL3A1, COL10A1, and COL11A1. (B) Correlations between the seven hub genes and clinical characteristics. The numbers in each small rectangle indicate the P-value for the correlation. (C) The relationship between the hub genes and clinical stage and T stage. *P < 0.05, **P < 0.01, ***P < 0.001.
    Immunohistochemistry (Ihc) Staining Images Of Col3a1, Spp1, And Thbs2, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ihc+staining+images+of+opn/spp1+protein/pmc07921701-64-9-21
    Average 90 stars, based on 1 article reviews
    immunohistochemistry (ihc) staining images of col3a1, spp1, and thbs2 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    High OPN expression in HCC. ( a ) The mRNA level of SPP1 in different types of tumor tissues and normal tissues in TIMER database. ( b ) Representative images of OPN protein level in HCC detected by IHC staining in HPA database (×400 magnification). ( c ) Comparison of OPN protein expression between HCC and normal tissues in CPTAC database. A two-sided p value was calculated using a Student’s t -test. The box plot indicates the median (center), 25th and 75th percentiles (box boundaries), and the minimum and maximum (whiskers). ( d ) Single-cell RNA sequencing localization analysis of SPP1. (Left) t-SNE plot of three main cell types (Macrophages, dendritic cells, and B cells) in hepatic monocyte-derived macrophages. (Right) SPP1 was enriched in macrophages clusters.

    Journal: Journal of Hepatocellular Carcinoma

    Article Title: Serum Osteopontin Enhances Hepatocellular Carcinoma Diagnosis and Predicts Anti-PD-L1 Immunotherapy Benefit

    doi: 10.2147/JHC.S514144

    Figure Lengend Snippet: High OPN expression in HCC. ( a ) The mRNA level of SPP1 in different types of tumor tissues and normal tissues in TIMER database. ( b ) Representative images of OPN protein level in HCC detected by IHC staining in HPA database (×400 magnification). ( c ) Comparison of OPN protein expression between HCC and normal tissues in CPTAC database. A two-sided p value was calculated using a Student’s t -test. The box plot indicates the median (center), 25th and 75th percentiles (box boundaries), and the minimum and maximum (whiskers). ( d ) Single-cell RNA sequencing localization analysis of SPP1. (Left) t-SNE plot of three main cell types (Macrophages, dendritic cells, and B cells) in hepatic monocyte-derived macrophages. (Right) SPP1 was enriched in macrophages clusters.

    Article Snippet: Immunohistochemistry (IHC) staining images of OPN in HCC and normal liver tissues were retrieved from the Human Protein Atlas (HPA; www.proteinatlas.org ) online database.

    Techniques: Expressing, Immunohistochemistry, Comparison, RNA Sequencing, Derivative Assay

    Diagnostic value of serum OPN for HCC. ( a – d ) Violin plots of serum OPN levels in healthy participants, CLD patients, and HCC patients ( a ), in early-stage HCC patients and advanced-stage HCC patients ( b ), in CLD patients and early-stage HCC patients ( c ), in CLD patients and AFP-negative HCC patients ( d ). ( e – g ) ROC curve analysis reveals the diagnostic potential of serum OPN in 178 HCC patients and 138 controls ( e ), in 98 early-stage HCC patients and 53 chronic liver disease patients ( f ), in 101 AFP-negative HCC patients and 53 chronic liver disease patients ( g ). ( h–j ) The waterfall plot illustrates the risk probability distribution between serum samples of patients with HCC (n = 178) and the controls (n = 138) in the whole cohort ( h ), patients with early-stage HCC (n = 98) and the chronic liver disease (n = 53) ( i ), patients with AFP-negative HCC (n = 101) and the chronic liver disease (n = 53) ( j ). ( k ) The rate of positive results for AFP, OPN, or both, in all patients with HCC, for OPN in AFP-negative HCC, and for OPN in early-stage HCC, respectively. ( l ) Comparison of ROC of four diagnostic models for distinguishing HCC from whole controls.

    Journal: Journal of Hepatocellular Carcinoma

    Article Title: Serum Osteopontin Enhances Hepatocellular Carcinoma Diagnosis and Predicts Anti-PD-L1 Immunotherapy Benefit

    doi: 10.2147/JHC.S514144

    Figure Lengend Snippet: Diagnostic value of serum OPN for HCC. ( a – d ) Violin plots of serum OPN levels in healthy participants, CLD patients, and HCC patients ( a ), in early-stage HCC patients and advanced-stage HCC patients ( b ), in CLD patients and early-stage HCC patients ( c ), in CLD patients and AFP-negative HCC patients ( d ). ( e – g ) ROC curve analysis reveals the diagnostic potential of serum OPN in 178 HCC patients and 138 controls ( e ), in 98 early-stage HCC patients and 53 chronic liver disease patients ( f ), in 101 AFP-negative HCC patients and 53 chronic liver disease patients ( g ). ( h–j ) The waterfall plot illustrates the risk probability distribution between serum samples of patients with HCC (n = 178) and the controls (n = 138) in the whole cohort ( h ), patients with early-stage HCC (n = 98) and the chronic liver disease (n = 53) ( i ), patients with AFP-negative HCC (n = 101) and the chronic liver disease (n = 53) ( j ). ( k ) The rate of positive results for AFP, OPN, or both, in all patients with HCC, for OPN in AFP-negative HCC, and for OPN in early-stage HCC, respectively. ( l ) Comparison of ROC of four diagnostic models for distinguishing HCC from whole controls.

    Article Snippet: Immunohistochemistry (IHC) staining images of OPN in HCC and normal liver tissues were retrieved from the Human Protein Atlas (HPA; www.proteinatlas.org ) online database.

    Techniques: Diagnostic Assay, Comparison

    Serum OPN as an independent predictor for HCC survival. ( a ) The inter-relationship between each clinical parameters in HCC patients. Values denote the Pearson correlation coefficients; values closer to 1 indicate a better correlation (* p < 0.05; ** p < 0.001). ( b ) Optimal cutoff value of serum OPN was determined by the “surv_cutpoint” function. ( c ) Forest plot of multivariate Cox regression analysis showing serum OPN level, hepatectomy, tumor size, Child-Pugh grade, and BCLC stage as independent prognostic factors for OS. ( d ) Forest plot of multivariate Cox regression analysis showing serum OPN level, Child-Pugh grade, and BCLC stage as independent prognostic factors for DFS. ( e ) Alluvial diagram showing the interrelationship between AFP status, OPN groups, and OS status in HCC. ( f ) Alluvial diagram showing the interrelationship between OPN groups, BCLC stage, and recurrence status in HCC patients.

    Journal: Journal of Hepatocellular Carcinoma

    Article Title: Serum Osteopontin Enhances Hepatocellular Carcinoma Diagnosis and Predicts Anti-PD-L1 Immunotherapy Benefit

    doi: 10.2147/JHC.S514144

    Figure Lengend Snippet: Serum OPN as an independent predictor for HCC survival. ( a ) The inter-relationship between each clinical parameters in HCC patients. Values denote the Pearson correlation coefficients; values closer to 1 indicate a better correlation (* p < 0.05; ** p < 0.001). ( b ) Optimal cutoff value of serum OPN was determined by the “surv_cutpoint” function. ( c ) Forest plot of multivariate Cox regression analysis showing serum OPN level, hepatectomy, tumor size, Child-Pugh grade, and BCLC stage as independent prognostic factors for OS. ( d ) Forest plot of multivariate Cox regression analysis showing serum OPN level, Child-Pugh grade, and BCLC stage as independent prognostic factors for DFS. ( e ) Alluvial diagram showing the interrelationship between AFP status, OPN groups, and OS status in HCC. ( f ) Alluvial diagram showing the interrelationship between OPN groups, BCLC stage, and recurrence status in HCC patients.

    Article Snippet: Immunohistochemistry (IHC) staining images of OPN in HCC and normal liver tissues were retrieved from the Human Protein Atlas (HPA; www.proteinatlas.org ) online database.

    Techniques:

    Correlation Between Serum OPN and Clinicopathologic Factors in HCC Patients

    Journal: Journal of Hepatocellular Carcinoma

    Article Title: Serum Osteopontin Enhances Hepatocellular Carcinoma Diagnosis and Predicts Anti-PD-L1 Immunotherapy Benefit

    doi: 10.2147/JHC.S514144

    Figure Lengend Snippet: Correlation Between Serum OPN and Clinicopathologic Factors in HCC Patients

    Article Snippet: Immunohistochemistry (IHC) staining images of OPN in HCC and normal liver tissues were retrieved from the Human Protein Atlas (HPA; www.proteinatlas.org ) online database.

    Techniques:

    Prognostic value of OPN in patients with HCC. ( a and b ) In the whole HCC cohort, patients with low levels of serum OPN had longer overall survival ( a ) and disease-free survival (b). ( c – i ) The prognostic value of serum OPN in different subgroups regarding overall survival, including early-stage ( c ), AFP-negative ( d ), Child-Pugh grade A ( e ), hepatectomy ( f ), tumor size ≤ 5 cm ( g ), MVI absent ( h ), ALBI grade 1 ( i ).

    Journal: Journal of Hepatocellular Carcinoma

    Article Title: Serum Osteopontin Enhances Hepatocellular Carcinoma Diagnosis and Predicts Anti-PD-L1 Immunotherapy Benefit

    doi: 10.2147/JHC.S514144

    Figure Lengend Snippet: Prognostic value of OPN in patients with HCC. ( a and b ) In the whole HCC cohort, patients with low levels of serum OPN had longer overall survival ( a ) and disease-free survival (b). ( c – i ) The prognostic value of serum OPN in different subgroups regarding overall survival, including early-stage ( c ), AFP-negative ( d ), Child-Pugh grade A ( e ), hepatectomy ( f ), tumor size ≤ 5 cm ( g ), MVI absent ( h ), ALBI grade 1 ( i ).

    Article Snippet: Immunohistochemistry (IHC) staining images of OPN in HCC and normal liver tissues were retrieved from the Human Protein Atlas (HPA; www.proteinatlas.org ) online database.

    Techniques:

    Serum OPN is a predictor of anti-PD-L1 immunotherapy benefit in patients with HCC. ( a and b ) Immunotherapy significantly prolonged the OS ( a ) and DFS ( b ) of HCC patients with high OPN levels. ( c and d ) HCC patients with low OPN levels did not derived a significant survival benefit from immunotherapy. ( e and f) OS ( e ) and DFS ( f ) of OPN high and OPN low patients in the immunotherapy group were not significantly different. ( g and h ) OPN high patients exhibited a significantly shorter OS ( g ) and DFS ( h ) than OPN low patients in the group without immunotherapy.

    Journal: Journal of Hepatocellular Carcinoma

    Article Title: Serum Osteopontin Enhances Hepatocellular Carcinoma Diagnosis and Predicts Anti-PD-L1 Immunotherapy Benefit

    doi: 10.2147/JHC.S514144

    Figure Lengend Snippet: Serum OPN is a predictor of anti-PD-L1 immunotherapy benefit in patients with HCC. ( a and b ) Immunotherapy significantly prolonged the OS ( a ) and DFS ( b ) of HCC patients with high OPN levels. ( c and d ) HCC patients with low OPN levels did not derived a significant survival benefit from immunotherapy. ( e and f) OS ( e ) and DFS ( f ) of OPN high and OPN low patients in the immunotherapy group were not significantly different. ( g and h ) OPN high patients exhibited a significantly shorter OS ( g ) and DFS ( h ) than OPN low patients in the group without immunotherapy.

    Article Snippet: Immunohistochemistry (IHC) staining images of OPN in HCC and normal liver tissues were retrieved from the Human Protein Atlas (HPA; www.proteinatlas.org ) online database.

    Techniques: Derivative Assay

    Clinical pathological indicators and prognostic characteristics of elevated extracellular matrix (ECM) subtype genes: Analysis of the correlations between ECM subtype gene expression and survival and clinical characteristics. (A) Survival analysis for the ECM subtype genes COMP, SPP1, THBS2, COL1A1, COL3A1, COL10A1, and COL11A1. (B) Correlations between the seven hub genes and clinical characteristics. The numbers in each small rectangle indicate the P-value for the correlation. (C) The relationship between the hub genes and clinical stage and T stage. *P < 0.05, **P < 0.01, ***P < 0.001.

    Journal: Frontiers in Oncology

    Article Title: Zinc Finger Protein CTCF Regulates Extracellular Matrix (ECM)-Related Gene Expression Associated With the Wnt Signaling Pathway in Gastric Cancer

    doi: 10.3389/fonc.2020.625633

    Figure Lengend Snippet: Clinical pathological indicators and prognostic characteristics of elevated extracellular matrix (ECM) subtype genes: Analysis of the correlations between ECM subtype gene expression and survival and clinical characteristics. (A) Survival analysis for the ECM subtype genes COMP, SPP1, THBS2, COL1A1, COL3A1, COL10A1, and COL11A1. (B) Correlations between the seven hub genes and clinical characteristics. The numbers in each small rectangle indicate the P-value for the correlation. (C) The relationship between the hub genes and clinical stage and T stage. *P < 0.05, **P < 0.01, ***P < 0.001.

    Article Snippet: The immunohistochemistry (IHC) staining images of COL3A1, SPP1, and THBS2 from GC tumor and normal gastric tissues were downloaded from the Human Protein Atlas (HPA) (HPA, RRID : SCR_006710) database ( http://www.proteinatlas.org/ ) for further analysis ( – ).

    Techniques: Gene Expression

    Exploration of the biological relevance of expression differences in the extracellular matrix (ECM) subtype genes: (A–D) Representative immunohistochemistry (IHC) images of ECM subtype genes in gastric cancer (GC) tissues and normal tissues [Human Protein Atlas (HPA) database]. The quantification results suggested that the protein levels of COL1A1, COL3A1, SPP1, and THBS2 were significantly increased in GC tumor tissues compared to normal gastric tissues. (E) Expression levels of the ECM subtype genes were determined in seven GC cell lines (aggressive cell lines: BGC823, HGC27, LMSU, and NCCStCK140; and less aggressive cell lines: FU97, MKN45, and AGS).

    Journal: Frontiers in Oncology

    Article Title: Zinc Finger Protein CTCF Regulates Extracellular Matrix (ECM)-Related Gene Expression Associated With the Wnt Signaling Pathway in Gastric Cancer

    doi: 10.3389/fonc.2020.625633

    Figure Lengend Snippet: Exploration of the biological relevance of expression differences in the extracellular matrix (ECM) subtype genes: (A–D) Representative immunohistochemistry (IHC) images of ECM subtype genes in gastric cancer (GC) tissues and normal tissues [Human Protein Atlas (HPA) database]. The quantification results suggested that the protein levels of COL1A1, COL3A1, SPP1, and THBS2 were significantly increased in GC tumor tissues compared to normal gastric tissues. (E) Expression levels of the ECM subtype genes were determined in seven GC cell lines (aggressive cell lines: BGC823, HGC27, LMSU, and NCCStCK140; and less aggressive cell lines: FU97, MKN45, and AGS).

    Article Snippet: The immunohistochemistry (IHC) staining images of COL3A1, SPP1, and THBS2 from GC tumor and normal gastric tissues were downloaded from the Human Protein Atlas (HPA) (HPA, RRID : SCR_006710) database ( http://www.proteinatlas.org/ ) for further analysis ( – ).

    Techniques: Expressing, Immunohistochemistry